艾普东生物

- 产品概况
  - BE-Doubleflow: is our most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device when a hypoxia environment is needed, for studying the effect of circulating particles (bacteria, immune system, circulating tumor cells) and for endothelium/epithelium barrier when no ALI is needed or when flux plays a role in both sides of the coculture.
    
    Culture Models
    
    • Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut or heart, among others.
    
    • Combine 3D and 2D cultures in a hypoxia environment where oxygen is supplied to the cells via the medium flowing in one of the channels.
    
    • Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses and many more in a single cell culture, or in a complex coculture.
    
    Device features
    • Easy to use: Be-Doubleflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.
    • Easy to connect: Be-Doubleflow is compatible with all microfluidic flow control systems (syringe, peristaltic pumps, pressure control systems, rocker systems…).
    • No unspecific absorption: Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows inmunohistochemistry with fluorescent detection.
    • Cell recovery: The cell cultures used in the Be-Doubleflow can be easily recover for further experimentation.
- 产品特性
  - 类别
    
    Microfluidic Chips
    
    *本产品仅限于专业人员的科学研究使用，不得用于食品、临床诊断或治疗。
- 产品应用
  - 应用说明
    
    BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).
    Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both type of cells simultaneously, circulating urine on the renal epithelium culture and blood like medium in the vascular culture.
- 关联文献

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BE-DOUBLEFLOW STANDARD 微流控芯片

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